Eucalyptus camaldulensis is a medicinal plant known for its phenolic constituents and antioxidant properties. This study aimed to tentatively characterize the phytochemical profile of its methanolic leaf extract and evaluate its antioxidant activity and effects on MCF-7 breast cancer cell viability. Metabolites were profiled using liquid chromatography–electrospray ionization–tandem mass spectrometry (LC–ESI–MS/MS). Antioxidant activity was assessed using the DPPH radical-scavenging assay, and cell viability was evaluated using the MTT assay. A total of 26 metabolites were tentatively annotated, including phenolic acids, flavonoids, flavonoid glycosides, and hydrolyzable tannins, particularly ellagitannin-related derivatives. The estimated DPPH IC₅₀ was approximately 135 µg/mL, obtained by linear extrapolation beyond the tested concentration range. The estimated MTT IC₅₀ was approximately 183 µg/mL, based on nonlinear regression of concentration–response data. The extract showed antioxidant activity and reduced MCF-7 cell viability at higher concentrations. However, the DPPH IC₅₀ should be interpreted cautiously because it was extrapolated beyond the tested range. Further studies involving compound isolation, structural confirmation, and additional cell models are needed to validate the tentative metabolite assignments and investigate the extract’s biological effects.
Kuti, A., & Mazarei, Z. (2026). LC–ESI–MS/MS-Based Tentative Phytochemical Profiling and Bioactivity Assessment of Eucalyptus camaldulensis Leaf Extract. Materials Chemistry Horizons, (), -. doi: 10.22128/mch.2026.3514.1075
MLA
Ahmad Kuti; Zeinab Mazarei. "LC–ESI–MS/MS-Based Tentative Phytochemical Profiling and Bioactivity Assessment of Eucalyptus camaldulensis Leaf Extract", Materials Chemistry Horizons, , , 2026, -. doi: 10.22128/mch.2026.3514.1075
HARVARD
Kuti, A., Mazarei, Z. (2026). 'LC–ESI–MS/MS-Based Tentative Phytochemical Profiling and Bioactivity Assessment of Eucalyptus camaldulensis Leaf Extract', Materials Chemistry Horizons, (), pp. -. doi: 10.22128/mch.2026.3514.1075
VANCOUVER
Kuti, A., Mazarei, Z. LC–ESI–MS/MS-Based Tentative Phytochemical Profiling and Bioactivity Assessment of Eucalyptus camaldulensis Leaf Extract. Materials Chemistry Horizons, 2026; (): -. doi: 10.22128/mch.2026.3514.1075
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